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dynamag-96 side skirted magnetic rack  (Thermo Fisher)


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    Structured Review

    Thermo Fisher dynamag-96 side skirted magnetic rack
    Dynamag 96 Side Skirted Magnetic Rack, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dynamag-96+side+skirted+magnet/dynamag+96+side+skirted+magnetic+rack/pmc12069562-65-0-6
    Average 90 stars, based on 1 article reviews
    dynamag-96 side skirted magnetic rack - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Recombinant:

    Article Title: Immune correlates of HIV-1 reservoir cell decline in early-treated infants
    Article Snippet: DynaMag-96 Side Skirted Magnet , ThermoFisher Scientific , 12027.

    Article Title: Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
    Article Snippet: Invitrogen DynaMag-96 side skirted magnet , Thermo Fisher Scientific , Cat# 12027.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: Animals Name Company Catalog Number Adiponectin-Cre mouse The Jackson Laboratory 28020 NuTRAP mouse The Jackson Laboratory 29899 Reagents & Materials Name Company Catalog Number 1.5 mL DNA-LoBind tubes Eppendorf 86–923 100 μm cell strainer Falcon 352–360 15 mL tubes VWR 525–1071 2X TD buffer Illumina 15027866 384-well PCR plate Applied biosystem 4483285 40 μm cell strainer Falcon 352–340 50 mL tubes VWR 525–1077 AMPure XP reagent (SPRI beads) Beckman Coulter A63881 Bioanalyzer High Sensitivity DNA kit Agilent Technologies 5067–4626 Clear adhesive film Applied biosystem 4306311 DNase/RNase-free distilled water Invitrogen 10977015 Dounce tissue grinder DWK Life Sciences 357542 DTT Sigma D9779 DynaMag-96 side skirted magnet Thermo Fishers 12027 FACS tubes Falcon 28719128 HEPES Boston BioProducts BBH-75 Hoechst 33342 Invitrogen 2134015 KCl (2 M) Boston BioProducts MT-252 Magnetic separation rack for PCR 8-tube strips EpiCypher 10–0008 MgCl 2 (1 M) Boston BioProducts MT-200 MinElute PCR purification kit Qiagen 28004 NEBNext High-Fidelity 2X PCR master mix BioLabs M0541S NP40 Thermo Fishers 28324 PCR 8-tube strip USA scientific 1402–4708 Protease inhibitor cocktail (100X) Thermo Fishers 78439 Qubit dsDNA HS assay kit Invitrogen Q32851 Sucrose Sigma S0389–1KG SYBR Green I (10,000X) Invitrogen S7563 TDE I enzyme Illumina 15027865 Instruments Name Company Catalog Number Flow cytometer BD Biosciences FACSAria TM Fusion Qubit fluorometer Invitrogen Q33226 Real-Time PCR system Thermo Fishers QuantStudio 5 Open in a separate window Table of Materials.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fishers , 12027.

    Article Title: Innate acting memory Th1 cells modulate heterologous diseases
    Article Snippet: Briefly, Agencourt RNAClean XP paramagnetic beads (Beckman Coulter) were used in combination with a DynaMag-96 side skirted magnet (Thermo Fisher). cDNA was generated with the SuperScript II Reverse Transcriptase Kit (Thermo Fisher) and amplified with HiFi HotStart PCR Mix (KAPA Biosystems).

    Article Title: Product and methods useful for modulating and evaluating immune responses
    Article Snippet: The lysate plate was transferred to a magnet (DynaMag-96 Side Skirted Magnet, Life Technologies), the supernatant was removed, and the beads were washed three times in 100 μL of 80% ethanol, with care being taken to avoid loss of beads during the washes.

    Article Title: Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fisher Scientific , 12027.

    Article Title: RNA methylation controls stress granule formation and function during erythropoiesis
    Article Snippet: End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25 0 C for 45 min. End-repaired DNA was purified using AMPure XP Beads (1.8X of the reaction volume) (Agencourt AMPure XP – PCR purification Beads, BeckmanCoulter, A63881) and separating beads using DynaMag-96 Side Skirted Magnet (Life Technologies, 12027).

    Plasmid Preparation:

    Article Title: Immune correlates of HIV-1 reservoir cell decline in early-treated infants
    Article Snippet: DynaMag-96 Side Skirted Magnet , ThermoFisher Scientific , 12027.

    Article Title: Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
    Article Snippet: Invitrogen DynaMag-96 side skirted magnet , Thermo Fisher Scientific , Cat# 12027.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: Animals Name Company Catalog Number Adiponectin-Cre mouse The Jackson Laboratory 28020 NuTRAP mouse The Jackson Laboratory 29899 Reagents & Materials Name Company Catalog Number 1.5 mL DNA-LoBind tubes Eppendorf 86–923 100 μm cell strainer Falcon 352–360 15 mL tubes VWR 525–1071 2X TD buffer Illumina 15027866 384-well PCR plate Applied biosystem 4483285 40 μm cell strainer Falcon 352–340 50 mL tubes VWR 525–1077 AMPure XP reagent (SPRI beads) Beckman Coulter A63881 Bioanalyzer High Sensitivity DNA kit Agilent Technologies 5067–4626 Clear adhesive film Applied biosystem 4306311 DNase/RNase-free distilled water Invitrogen 10977015 Dounce tissue grinder DWK Life Sciences 357542 DTT Sigma D9779 DynaMag-96 side skirted magnet Thermo Fishers 12027 FACS tubes Falcon 28719128 HEPES Boston BioProducts BBH-75 Hoechst 33342 Invitrogen 2134015 KCl (2 M) Boston BioProducts MT-252 Magnetic separation rack for PCR 8-tube strips EpiCypher 10–0008 MgCl 2 (1 M) Boston BioProducts MT-200 MinElute PCR purification kit Qiagen 28004 NEBNext High-Fidelity 2X PCR master mix BioLabs M0541S NP40 Thermo Fishers 28324 PCR 8-tube strip USA scientific 1402–4708 Protease inhibitor cocktail (100X) Thermo Fishers 78439 Qubit dsDNA HS assay kit Invitrogen Q32851 Sucrose Sigma S0389–1KG SYBR Green I (10,000X) Invitrogen S7563 TDE I enzyme Illumina 15027865 Instruments Name Company Catalog Number Flow cytometer BD Biosciences FACSAria TM Fusion Qubit fluorometer Invitrogen Q33226 Real-Time PCR system Thermo Fishers QuantStudio 5 Open in a separate window Table of Materials.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fishers , 12027.

    Article Title: Innate acting memory Th1 cells modulate heterologous diseases
    Article Snippet: Briefly, Agencourt RNAClean XP paramagnetic beads (Beckman Coulter) were used in combination with a DynaMag-96 side skirted magnet (Thermo Fisher). cDNA was generated with the SuperScript II Reverse Transcriptase Kit (Thermo Fisher) and amplified with HiFi HotStart PCR Mix (KAPA Biosystems).

    Article Title: Product and methods useful for modulating and evaluating immune responses
    Article Snippet: The lysate plate was transferred to a magnet (DynaMag-96 Side Skirted Magnet, Life Technologies), the supernatant was removed, and the beads were washed three times in 100 μL of 80% ethanol, with care being taken to avoid loss of beads during the washes.

    Article Title: Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fisher Scientific , 12027.

    Article Title: RNA methylation controls stress granule formation and function during erythropoiesis
    Article Snippet: End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25 0 C for 45 min. End-repaired DNA was purified using AMPure XP Beads (1.8X of the reaction volume) (Agencourt AMPure XP – PCR purification Beads, BeckmanCoulter, A63881) and separating beads using DynaMag-96 Side Skirted Magnet (Life Technologies, 12027).

    Reverse Transcription:

    Article Title: Immune correlates of HIV-1 reservoir cell decline in early-treated infants
    Article Snippet: DynaMag-96 Side Skirted Magnet , ThermoFisher Scientific , 12027.

    Article Title: Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
    Article Snippet: Invitrogen DynaMag-96 side skirted magnet , Thermo Fisher Scientific , Cat# 12027.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: Animals Name Company Catalog Number Adiponectin-Cre mouse The Jackson Laboratory 28020 NuTRAP mouse The Jackson Laboratory 29899 Reagents & Materials Name Company Catalog Number 1.5 mL DNA-LoBind tubes Eppendorf 86–923 100 μm cell strainer Falcon 352–360 15 mL tubes VWR 525–1071 2X TD buffer Illumina 15027866 384-well PCR plate Applied biosystem 4483285 40 μm cell strainer Falcon 352–340 50 mL tubes VWR 525–1077 AMPure XP reagent (SPRI beads) Beckman Coulter A63881 Bioanalyzer High Sensitivity DNA kit Agilent Technologies 5067–4626 Clear adhesive film Applied biosystem 4306311 DNase/RNase-free distilled water Invitrogen 10977015 Dounce tissue grinder DWK Life Sciences 357542 DTT Sigma D9779 DynaMag-96 side skirted magnet Thermo Fishers 12027 FACS tubes Falcon 28719128 HEPES Boston BioProducts BBH-75 Hoechst 33342 Invitrogen 2134015 KCl (2 M) Boston BioProducts MT-252 Magnetic separation rack for PCR 8-tube strips EpiCypher 10–0008 MgCl 2 (1 M) Boston BioProducts MT-200 MinElute PCR purification kit Qiagen 28004 NEBNext High-Fidelity 2X PCR master mix BioLabs M0541S NP40 Thermo Fishers 28324 PCR 8-tube strip USA scientific 1402–4708 Protease inhibitor cocktail (100X) Thermo Fishers 78439 Qubit dsDNA HS assay kit Invitrogen Q32851 Sucrose Sigma S0389–1KG SYBR Green I (10,000X) Invitrogen S7563 TDE I enzyme Illumina 15027865 Instruments Name Company Catalog Number Flow cytometer BD Biosciences FACSAria TM Fusion Qubit fluorometer Invitrogen Q33226 Real-Time PCR system Thermo Fishers QuantStudio 5 Open in a separate window Table of Materials.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fishers , 12027.

    Article Title: Innate acting memory Th1 cells modulate heterologous diseases
    Article Snippet: Briefly, Agencourt RNAClean XP paramagnetic beads (Beckman Coulter) were used in combination with a DynaMag-96 side skirted magnet (Thermo Fisher). cDNA was generated with the SuperScript II Reverse Transcriptase Kit (Thermo Fisher) and amplified with HiFi HotStart PCR Mix (KAPA Biosystems).

    Article Title: Product and methods useful for modulating and evaluating immune responses
    Article Snippet: The lysate plate was transferred to a magnet (DynaMag-96 Side Skirted Magnet, Life Technologies), the supernatant was removed, and the beads were washed three times in 100 μL of 80% ethanol, with care being taken to avoid loss of beads during the washes.

    Article Title: Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fisher Scientific , 12027.

    Article Title: RNA methylation controls stress granule formation and function during erythropoiesis
    Article Snippet: End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25 0 C for 45 min. End-repaired DNA was purified using AMPure XP Beads (1.8X of the reaction volume) (Agencourt AMPure XP – PCR purification Beads, BeckmanCoulter, A63881) and separating beads using DynaMag-96 Side Skirted Magnet (Life Technologies, 12027).

    DNA Ligation:

    Article Title: Immune correlates of HIV-1 reservoir cell decline in early-treated infants
    Article Snippet: DynaMag-96 Side Skirted Magnet , ThermoFisher Scientific , 12027.

    Article Title: Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
    Article Snippet: Invitrogen DynaMag-96 side skirted magnet , Thermo Fisher Scientific , Cat# 12027.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: Animals Name Company Catalog Number Adiponectin-Cre mouse The Jackson Laboratory 28020 NuTRAP mouse The Jackson Laboratory 29899 Reagents & Materials Name Company Catalog Number 1.5 mL DNA-LoBind tubes Eppendorf 86–923 100 μm cell strainer Falcon 352–360 15 mL tubes VWR 525–1071 2X TD buffer Illumina 15027866 384-well PCR plate Applied biosystem 4483285 40 μm cell strainer Falcon 352–340 50 mL tubes VWR 525–1077 AMPure XP reagent (SPRI beads) Beckman Coulter A63881 Bioanalyzer High Sensitivity DNA kit Agilent Technologies 5067–4626 Clear adhesive film Applied biosystem 4306311 DNase/RNase-free distilled water Invitrogen 10977015 Dounce tissue grinder DWK Life Sciences 357542 DTT Sigma D9779 DynaMag-96 side skirted magnet Thermo Fishers 12027 FACS tubes Falcon 28719128 HEPES Boston BioProducts BBH-75 Hoechst 33342 Invitrogen 2134015 KCl (2 M) Boston BioProducts MT-252 Magnetic separation rack for PCR 8-tube strips EpiCypher 10–0008 MgCl 2 (1 M) Boston BioProducts MT-200 MinElute PCR purification kit Qiagen 28004 NEBNext High-Fidelity 2X PCR master mix BioLabs M0541S NP40 Thermo Fishers 28324 PCR 8-tube strip USA scientific 1402–4708 Protease inhibitor cocktail (100X) Thermo Fishers 78439 Qubit dsDNA HS assay kit Invitrogen Q32851 Sucrose Sigma S0389–1KG SYBR Green I (10,000X) Invitrogen S7563 TDE I enzyme Illumina 15027865 Instruments Name Company Catalog Number Flow cytometer BD Biosciences FACSAria TM Fusion Qubit fluorometer Invitrogen Q33226 Real-Time PCR system Thermo Fishers QuantStudio 5 Open in a separate window Table of Materials.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fishers , 12027.

    Article Title: Innate acting memory Th1 cells modulate heterologous diseases
    Article Snippet: Briefly, Agencourt RNAClean XP paramagnetic beads (Beckman Coulter) were used in combination with a DynaMag-96 side skirted magnet (Thermo Fisher). cDNA was generated with the SuperScript II Reverse Transcriptase Kit (Thermo Fisher) and amplified with HiFi HotStart PCR Mix (KAPA Biosystems).

    Article Title: Product and methods useful for modulating and evaluating immune responses
    Article Snippet: The lysate plate was transferred to a magnet (DynaMag-96 Side Skirted Magnet, Life Technologies), the supernatant was removed, and the beads were washed three times in 100 μL of 80% ethanol, with care being taken to avoid loss of beads during the washes.

    Article Title: Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fisher Scientific , 12027.

    Article Title: RNA methylation controls stress granule formation and function during erythropoiesis
    Article Snippet: End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25 0 C for 45 min. End-repaired DNA was purified using AMPure XP Beads (1.8X of the reaction volume) (Agencourt AMPure XP – PCR purification Beads, BeckmanCoulter, A63881) and separating beads using DynaMag-96 Side Skirted Magnet (Life Technologies, 12027).

    Library Quantification:

    Article Title: Immune correlates of HIV-1 reservoir cell decline in early-treated infants
    Article Snippet: DynaMag-96 Side Skirted Magnet , ThermoFisher Scientific , 12027.

    Article Title: Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
    Article Snippet: Invitrogen DynaMag-96 side skirted magnet , Thermo Fisher Scientific , Cat# 12027.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: Animals Name Company Catalog Number Adiponectin-Cre mouse The Jackson Laboratory 28020 NuTRAP mouse The Jackson Laboratory 29899 Reagents & Materials Name Company Catalog Number 1.5 mL DNA-LoBind tubes Eppendorf 86–923 100 μm cell strainer Falcon 352–360 15 mL tubes VWR 525–1071 2X TD buffer Illumina 15027866 384-well PCR plate Applied biosystem 4483285 40 μm cell strainer Falcon 352–340 50 mL tubes VWR 525–1077 AMPure XP reagent (SPRI beads) Beckman Coulter A63881 Bioanalyzer High Sensitivity DNA kit Agilent Technologies 5067–4626 Clear adhesive film Applied biosystem 4306311 DNase/RNase-free distilled water Invitrogen 10977015 Dounce tissue grinder DWK Life Sciences 357542 DTT Sigma D9779 DynaMag-96 side skirted magnet Thermo Fishers 12027 FACS tubes Falcon 28719128 HEPES Boston BioProducts BBH-75 Hoechst 33342 Invitrogen 2134015 KCl (2 M) Boston BioProducts MT-252 Magnetic separation rack for PCR 8-tube strips EpiCypher 10–0008 MgCl 2 (1 M) Boston BioProducts MT-200 MinElute PCR purification kit Qiagen 28004 NEBNext High-Fidelity 2X PCR master mix BioLabs M0541S NP40 Thermo Fishers 28324 PCR 8-tube strip USA scientific 1402–4708 Protease inhibitor cocktail (100X) Thermo Fishers 78439 Qubit dsDNA HS assay kit Invitrogen Q32851 Sucrose Sigma S0389–1KG SYBR Green I (10,000X) Invitrogen S7563 TDE I enzyme Illumina 15027865 Instruments Name Company Catalog Number Flow cytometer BD Biosciences FACSAria TM Fusion Qubit fluorometer Invitrogen Q33226 Real-Time PCR system Thermo Fishers QuantStudio 5 Open in a separate window Table of Materials.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fishers , 12027.

    Article Title: Innate acting memory Th1 cells modulate heterologous diseases
    Article Snippet: Briefly, Agencourt RNAClean XP paramagnetic beads (Beckman Coulter) were used in combination with a DynaMag-96 side skirted magnet (Thermo Fisher). cDNA was generated with the SuperScript II Reverse Transcriptase Kit (Thermo Fisher) and amplified with HiFi HotStart PCR Mix (KAPA Biosystems).

    Article Title: Product and methods useful for modulating and evaluating immune responses
    Article Snippet: The lysate plate was transferred to a magnet (DynaMag-96 Side Skirted Magnet, Life Technologies), the supernatant was removed, and the beads were washed three times in 100 μL of 80% ethanol, with care being taken to avoid loss of beads during the washes.

    Article Title: Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fisher Scientific , 12027.

    Article Title: RNA methylation controls stress granule formation and function during erythropoiesis
    Article Snippet: End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25 0 C for 45 min. End-repaired DNA was purified using AMPure XP Beads (1.8X of the reaction volume) (Agencourt AMPure XP – PCR purification Beads, BeckmanCoulter, A63881) and separating beads using DynaMag-96 Side Skirted Magnet (Life Technologies, 12027).

    Sequencing:

    Article Title: Immune correlates of HIV-1 reservoir cell decline in early-treated infants
    Article Snippet: DynaMag-96 Side Skirted Magnet , ThermoFisher Scientific , 12027.

    Article Title: Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
    Article Snippet: Invitrogen DynaMag-96 side skirted magnet , Thermo Fisher Scientific , Cat# 12027.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: Animals Name Company Catalog Number Adiponectin-Cre mouse The Jackson Laboratory 28020 NuTRAP mouse The Jackson Laboratory 29899 Reagents & Materials Name Company Catalog Number 1.5 mL DNA-LoBind tubes Eppendorf 86–923 100 μm cell strainer Falcon 352–360 15 mL tubes VWR 525–1071 2X TD buffer Illumina 15027866 384-well PCR plate Applied biosystem 4483285 40 μm cell strainer Falcon 352–340 50 mL tubes VWR 525–1077 AMPure XP reagent (SPRI beads) Beckman Coulter A63881 Bioanalyzer High Sensitivity DNA kit Agilent Technologies 5067–4626 Clear adhesive film Applied biosystem 4306311 DNase/RNase-free distilled water Invitrogen 10977015 Dounce tissue grinder DWK Life Sciences 357542 DTT Sigma D9779 DynaMag-96 side skirted magnet Thermo Fishers 12027 FACS tubes Falcon 28719128 HEPES Boston BioProducts BBH-75 Hoechst 33342 Invitrogen 2134015 KCl (2 M) Boston BioProducts MT-252 Magnetic separation rack for PCR 8-tube strips EpiCypher 10–0008 MgCl 2 (1 M) Boston BioProducts MT-200 MinElute PCR purification kit Qiagen 28004 NEBNext High-Fidelity 2X PCR master mix BioLabs M0541S NP40 Thermo Fishers 28324 PCR 8-tube strip USA scientific 1402–4708 Protease inhibitor cocktail (100X) Thermo Fishers 78439 Qubit dsDNA HS assay kit Invitrogen Q32851 Sucrose Sigma S0389–1KG SYBR Green I (10,000X) Invitrogen S7563 TDE I enzyme Illumina 15027865 Instruments Name Company Catalog Number Flow cytometer BD Biosciences FACSAria TM Fusion Qubit fluorometer Invitrogen Q33226 Real-Time PCR system Thermo Fishers QuantStudio 5 Open in a separate window Table of Materials.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fishers , 12027.

    Article Title: Innate acting memory Th1 cells modulate heterologous diseases
    Article Snippet: Briefly, Agencourt RNAClean XP paramagnetic beads (Beckman Coulter) were used in combination with a DynaMag-96 side skirted magnet (Thermo Fisher). cDNA was generated with the SuperScript II Reverse Transcriptase Kit (Thermo Fisher) and amplified with HiFi HotStart PCR Mix (KAPA Biosystems).

    Article Title: Product and methods useful for modulating and evaluating immune responses
    Article Snippet: The lysate plate was transferred to a magnet (DynaMag-96 Side Skirted Magnet, Life Technologies), the supernatant was removed, and the beads were washed three times in 100 μL of 80% ethanol, with care being taken to avoid loss of beads during the washes.

    Article Title: Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fisher Scientific , 12027.

    Article Title: RNA methylation controls stress granule formation and function during erythropoiesis
    Article Snippet: End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25 0 C for 45 min. End-repaired DNA was purified using AMPure XP Beads (1.8X of the reaction volume) (Agencourt AMPure XP – PCR purification Beads, BeckmanCoulter, A63881) and separating beads using DynaMag-96 Side Skirted Magnet (Life Technologies, 12027).

    Software:

    Article Title: Immune correlates of HIV-1 reservoir cell decline in early-treated infants
    Article Snippet: DynaMag-96 Side Skirted Magnet , ThermoFisher Scientific , 12027.

    Article Title: Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
    Article Snippet: Invitrogen DynaMag-96 side skirted magnet , Thermo Fisher Scientific , Cat# 12027.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: Animals Name Company Catalog Number Adiponectin-Cre mouse The Jackson Laboratory 28020 NuTRAP mouse The Jackson Laboratory 29899 Reagents & Materials Name Company Catalog Number 1.5 mL DNA-LoBind tubes Eppendorf 86–923 100 μm cell strainer Falcon 352–360 15 mL tubes VWR 525–1071 2X TD buffer Illumina 15027866 384-well PCR plate Applied biosystem 4483285 40 μm cell strainer Falcon 352–340 50 mL tubes VWR 525–1077 AMPure XP reagent (SPRI beads) Beckman Coulter A63881 Bioanalyzer High Sensitivity DNA kit Agilent Technologies 5067–4626 Clear adhesive film Applied biosystem 4306311 DNase/RNase-free distilled water Invitrogen 10977015 Dounce tissue grinder DWK Life Sciences 357542 DTT Sigma D9779 DynaMag-96 side skirted magnet Thermo Fishers 12027 FACS tubes Falcon 28719128 HEPES Boston BioProducts BBH-75 Hoechst 33342 Invitrogen 2134015 KCl (2 M) Boston BioProducts MT-252 Magnetic separation rack for PCR 8-tube strips EpiCypher 10–0008 MgCl 2 (1 M) Boston BioProducts MT-200 MinElute PCR purification kit Qiagen 28004 NEBNext High-Fidelity 2X PCR master mix BioLabs M0541S NP40 Thermo Fishers 28324 PCR 8-tube strip USA scientific 1402–4708 Protease inhibitor cocktail (100X) Thermo Fishers 78439 Qubit dsDNA HS assay kit Invitrogen Q32851 Sucrose Sigma S0389–1KG SYBR Green I (10,000X) Invitrogen S7563 TDE I enzyme Illumina 15027865 Instruments Name Company Catalog Number Flow cytometer BD Biosciences FACSAria TM Fusion Qubit fluorometer Invitrogen Q33226 Real-Time PCR system Thermo Fishers QuantStudio 5 Open in a separate window Table of Materials.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fishers , 12027.

    Article Title: Innate acting memory Th1 cells modulate heterologous diseases
    Article Snippet: Briefly, Agencourt RNAClean XP paramagnetic beads (Beckman Coulter) were used in combination with a DynaMag-96 side skirted magnet (Thermo Fisher). cDNA was generated with the SuperScript II Reverse Transcriptase Kit (Thermo Fisher) and amplified with HiFi HotStart PCR Mix (KAPA Biosystems).

    Article Title: Product and methods useful for modulating and evaluating immune responses
    Article Snippet: The lysate plate was transferred to a magnet (DynaMag-96 Side Skirted Magnet, Life Technologies), the supernatant was removed, and the beads were washed three times in 100 μL of 80% ethanol, with care being taken to avoid loss of beads during the washes.

    Article Title: Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fisher Scientific , 12027.

    Article Title: RNA methylation controls stress granule formation and function during erythropoiesis
    Article Snippet: End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25 0 C for 45 min. End-repaired DNA was purified using AMPure XP Beads (1.8X of the reaction volume) (Agencourt AMPure XP – PCR purification Beads, BeckmanCoulter, A63881) and separating beads using DynaMag-96 Side Skirted Magnet (Life Technologies, 12027).

    Sterility:

    Article Title: Immune correlates of HIV-1 reservoir cell decline in early-treated infants
    Article Snippet: DynaMag-96 Side Skirted Magnet , ThermoFisher Scientific , 12027.

    Article Title: Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
    Article Snippet: Invitrogen DynaMag-96 side skirted magnet , Thermo Fisher Scientific , Cat# 12027.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: Animals Name Company Catalog Number Adiponectin-Cre mouse The Jackson Laboratory 28020 NuTRAP mouse The Jackson Laboratory 29899 Reagents & Materials Name Company Catalog Number 1.5 mL DNA-LoBind tubes Eppendorf 86–923 100 μm cell strainer Falcon 352–360 15 mL tubes VWR 525–1071 2X TD buffer Illumina 15027866 384-well PCR plate Applied biosystem 4483285 40 μm cell strainer Falcon 352–340 50 mL tubes VWR 525–1077 AMPure XP reagent (SPRI beads) Beckman Coulter A63881 Bioanalyzer High Sensitivity DNA kit Agilent Technologies 5067–4626 Clear adhesive film Applied biosystem 4306311 DNase/RNase-free distilled water Invitrogen 10977015 Dounce tissue grinder DWK Life Sciences 357542 DTT Sigma D9779 DynaMag-96 side skirted magnet Thermo Fishers 12027 FACS tubes Falcon 28719128 HEPES Boston BioProducts BBH-75 Hoechst 33342 Invitrogen 2134015 KCl (2 M) Boston BioProducts MT-252 Magnetic separation rack for PCR 8-tube strips EpiCypher 10–0008 MgCl 2 (1 M) Boston BioProducts MT-200 MinElute PCR purification kit Qiagen 28004 NEBNext High-Fidelity 2X PCR master mix BioLabs M0541S NP40 Thermo Fishers 28324 PCR 8-tube strip USA scientific 1402–4708 Protease inhibitor cocktail (100X) Thermo Fishers 78439 Qubit dsDNA HS assay kit Invitrogen Q32851 Sucrose Sigma S0389–1KG SYBR Green I (10,000X) Invitrogen S7563 TDE I enzyme Illumina 15027865 Instruments Name Company Catalog Number Flow cytometer BD Biosciences FACSAria TM Fusion Qubit fluorometer Invitrogen Q33226 Real-Time PCR system Thermo Fishers QuantStudio 5 Open in a separate window Table of Materials.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fishers , 12027.

    Article Title: Innate acting memory Th1 cells modulate heterologous diseases
    Article Snippet: Briefly, Agencourt RNAClean XP paramagnetic beads (Beckman Coulter) were used in combination with a DynaMag-96 side skirted magnet (Thermo Fisher). cDNA was generated with the SuperScript II Reverse Transcriptase Kit (Thermo Fisher) and amplified with HiFi HotStart PCR Mix (KAPA Biosystems).

    Article Title: Product and methods useful for modulating and evaluating immune responses
    Article Snippet: The lysate plate was transferred to a magnet (DynaMag-96 Side Skirted Magnet, Life Technologies), the supernatant was removed, and the beads were washed three times in 100 μL of 80% ethanol, with care being taken to avoid loss of beads during the washes.

    Article Title: Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fisher Scientific , 12027.

    Article Title: RNA methylation controls stress granule formation and function during erythropoiesis
    Article Snippet: End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25 0 C for 45 min. End-repaired DNA was purified using AMPure XP Beads (1.8X of the reaction volume) (Agencourt AMPure XP – PCR purification Beads, BeckmanCoulter, A63881) and separating beads using DynaMag-96 Side Skirted Magnet (Life Technologies, 12027).

    Adhesive:

    Article Title: Immune correlates of HIV-1 reservoir cell decline in early-treated infants
    Article Snippet: DynaMag-96 Side Skirted Magnet , ThermoFisher Scientific , 12027.

    Article Title: Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
    Article Snippet: Invitrogen DynaMag-96 side skirted magnet , Thermo Fisher Scientific , Cat# 12027.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: Animals Name Company Catalog Number Adiponectin-Cre mouse The Jackson Laboratory 28020 NuTRAP mouse The Jackson Laboratory 29899 Reagents & Materials Name Company Catalog Number 1.5 mL DNA-LoBind tubes Eppendorf 86–923 100 μm cell strainer Falcon 352–360 15 mL tubes VWR 525–1071 2X TD buffer Illumina 15027866 384-well PCR plate Applied biosystem 4483285 40 μm cell strainer Falcon 352–340 50 mL tubes VWR 525–1077 AMPure XP reagent (SPRI beads) Beckman Coulter A63881 Bioanalyzer High Sensitivity DNA kit Agilent Technologies 5067–4626 Clear adhesive film Applied biosystem 4306311 DNase/RNase-free distilled water Invitrogen 10977015 Dounce tissue grinder DWK Life Sciences 357542 DTT Sigma D9779 DynaMag-96 side skirted magnet Thermo Fishers 12027 FACS tubes Falcon 28719128 HEPES Boston BioProducts BBH-75 Hoechst 33342 Invitrogen 2134015 KCl (2 M) Boston BioProducts MT-252 Magnetic separation rack for PCR 8-tube strips EpiCypher 10–0008 MgCl 2 (1 M) Boston BioProducts MT-200 MinElute PCR purification kit Qiagen 28004 NEBNext High-Fidelity 2X PCR master mix BioLabs M0541S NP40 Thermo Fishers 28324 PCR 8-tube strip USA scientific 1402–4708 Protease inhibitor cocktail (100X) Thermo Fishers 78439 Qubit dsDNA HS assay kit Invitrogen Q32851 Sucrose Sigma S0389–1KG SYBR Green I (10,000X) Invitrogen S7563 TDE I enzyme Illumina 15027865 Instruments Name Company Catalog Number Flow cytometer BD Biosciences FACSAria TM Fusion Qubit fluorometer Invitrogen Q33226 Real-Time PCR system Thermo Fishers QuantStudio 5 Open in a separate window Table of Materials.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fishers , 12027.

    Article Title: Innate acting memory Th1 cells modulate heterologous diseases
    Article Snippet: Briefly, Agencourt RNAClean XP paramagnetic beads (Beckman Coulter) were used in combination with a DynaMag-96 side skirted magnet (Thermo Fisher). cDNA was generated with the SuperScript II Reverse Transcriptase Kit (Thermo Fisher) and amplified with HiFi HotStart PCR Mix (KAPA Biosystems).

    Article Title: Product and methods useful for modulating and evaluating immune responses
    Article Snippet: The lysate plate was transferred to a magnet (DynaMag-96 Side Skirted Magnet, Life Technologies), the supernatant was removed, and the beads were washed three times in 100 μL of 80% ethanol, with care being taken to avoid loss of beads during the washes.

    Article Title: Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fisher Scientific , 12027.

    Article Title: RNA methylation controls stress granule formation and function during erythropoiesis
    Article Snippet: End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25 0 C for 45 min. End-repaired DNA was purified using AMPure XP Beads (1.8X of the reaction volume) (Agencourt AMPure XP – PCR purification Beads, BeckmanCoulter, A63881) and separating beads using DynaMag-96 Side Skirted Magnet (Life Technologies, 12027).

    Immunoprecipitation:

    Article Title: Immune correlates of HIV-1 reservoir cell decline in early-treated infants
    Article Snippet: DynaMag-96 Side Skirted Magnet , ThermoFisher Scientific , 12027.

    Article Title: Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
    Article Snippet: Invitrogen DynaMag-96 side skirted magnet , Thermo Fisher Scientific , Cat# 12027.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: Animals Name Company Catalog Number Adiponectin-Cre mouse The Jackson Laboratory 28020 NuTRAP mouse The Jackson Laboratory 29899 Reagents & Materials Name Company Catalog Number 1.5 mL DNA-LoBind tubes Eppendorf 86–923 100 μm cell strainer Falcon 352–360 15 mL tubes VWR 525–1071 2X TD buffer Illumina 15027866 384-well PCR plate Applied biosystem 4483285 40 μm cell strainer Falcon 352–340 50 mL tubes VWR 525–1077 AMPure XP reagent (SPRI beads) Beckman Coulter A63881 Bioanalyzer High Sensitivity DNA kit Agilent Technologies 5067–4626 Clear adhesive film Applied biosystem 4306311 DNase/RNase-free distilled water Invitrogen 10977015 Dounce tissue grinder DWK Life Sciences 357542 DTT Sigma D9779 DynaMag-96 side skirted magnet Thermo Fishers 12027 FACS tubes Falcon 28719128 HEPES Boston BioProducts BBH-75 Hoechst 33342 Invitrogen 2134015 KCl (2 M) Boston BioProducts MT-252 Magnetic separation rack for PCR 8-tube strips EpiCypher 10–0008 MgCl 2 (1 M) Boston BioProducts MT-200 MinElute PCR purification kit Qiagen 28004 NEBNext High-Fidelity 2X PCR master mix BioLabs M0541S NP40 Thermo Fishers 28324 PCR 8-tube strip USA scientific 1402–4708 Protease inhibitor cocktail (100X) Thermo Fishers 78439 Qubit dsDNA HS assay kit Invitrogen Q32851 Sucrose Sigma S0389–1KG SYBR Green I (10,000X) Invitrogen S7563 TDE I enzyme Illumina 15027865 Instruments Name Company Catalog Number Flow cytometer BD Biosciences FACSAria TM Fusion Qubit fluorometer Invitrogen Q33226 Real-Time PCR system Thermo Fishers QuantStudio 5 Open in a separate window Table of Materials.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fishers , 12027.

    Article Title: Innate acting memory Th1 cells modulate heterologous diseases
    Article Snippet: Briefly, Agencourt RNAClean XP paramagnetic beads (Beckman Coulter) were used in combination with a DynaMag-96 side skirted magnet (Thermo Fisher). cDNA was generated with the SuperScript II Reverse Transcriptase Kit (Thermo Fisher) and amplified with HiFi HotStart PCR Mix (KAPA Biosystems).

    Article Title: Product and methods useful for modulating and evaluating immune responses
    Article Snippet: The lysate plate was transferred to a magnet (DynaMag-96 Side Skirted Magnet, Life Technologies), the supernatant was removed, and the beads were washed three times in 100 μL of 80% ethanol, with care being taken to avoid loss of beads during the washes.

    Article Title: Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fisher Scientific , 12027.

    Article Title: RNA methylation controls stress granule formation and function during erythropoiesis
    Article Snippet: End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25 0 C for 45 min. End-repaired DNA was purified using AMPure XP Beads (1.8X of the reaction volume) (Agencourt AMPure XP – PCR purification Beads, BeckmanCoulter, A63881) and separating beads using DynaMag-96 Side Skirted Magnet (Life Technologies, 12027).

    Purification:

    Article Title: Immune correlates of HIV-1 reservoir cell decline in early-treated infants
    Article Snippet: DynaMag-96 Side Skirted Magnet , ThermoFisher Scientific , 12027.

    Article Title: Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
    Article Snippet: Invitrogen DynaMag-96 side skirted magnet , Thermo Fisher Scientific , Cat# 12027.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: Animals Name Company Catalog Number Adiponectin-Cre mouse The Jackson Laboratory 28020 NuTRAP mouse The Jackson Laboratory 29899 Reagents & Materials Name Company Catalog Number 1.5 mL DNA-LoBind tubes Eppendorf 86–923 100 μm cell strainer Falcon 352–360 15 mL tubes VWR 525–1071 2X TD buffer Illumina 15027866 384-well PCR plate Applied biosystem 4483285 40 μm cell strainer Falcon 352–340 50 mL tubes VWR 525–1077 AMPure XP reagent (SPRI beads) Beckman Coulter A63881 Bioanalyzer High Sensitivity DNA kit Agilent Technologies 5067–4626 Clear adhesive film Applied biosystem 4306311 DNase/RNase-free distilled water Invitrogen 10977015 Dounce tissue grinder DWK Life Sciences 357542 DTT Sigma D9779 DynaMag-96 side skirted magnet Thermo Fishers 12027 FACS tubes Falcon 28719128 HEPES Boston BioProducts BBH-75 Hoechst 33342 Invitrogen 2134015 KCl (2 M) Boston BioProducts MT-252 Magnetic separation rack for PCR 8-tube strips EpiCypher 10–0008 MgCl 2 (1 M) Boston BioProducts MT-200 MinElute PCR purification kit Qiagen 28004 NEBNext High-Fidelity 2X PCR master mix BioLabs M0541S NP40 Thermo Fishers 28324 PCR 8-tube strip USA scientific 1402–4708 Protease inhibitor cocktail (100X) Thermo Fishers 78439 Qubit dsDNA HS assay kit Invitrogen Q32851 Sucrose Sigma S0389–1KG SYBR Green I (10,000X) Invitrogen S7563 TDE I enzyme Illumina 15027865 Instruments Name Company Catalog Number Flow cytometer BD Biosciences FACSAria TM Fusion Qubit fluorometer Invitrogen Q33226 Real-Time PCR system Thermo Fishers QuantStudio 5 Open in a separate window Table of Materials.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fishers , 12027.

    Article Title: Innate acting memory Th1 cells modulate heterologous diseases
    Article Snippet: Briefly, Agencourt RNAClean XP paramagnetic beads (Beckman Coulter) were used in combination with a DynaMag-96 side skirted magnet (Thermo Fisher). cDNA was generated with the SuperScript II Reverse Transcriptase Kit (Thermo Fisher) and amplified with HiFi HotStart PCR Mix (KAPA Biosystems).

    Article Title: Product and methods useful for modulating and evaluating immune responses
    Article Snippet: The lysate plate was transferred to a magnet (DynaMag-96 Side Skirted Magnet, Life Technologies), the supernatant was removed, and the beads were washed three times in 100 μL of 80% ethanol, with care being taken to avoid loss of beads during the washes.

    Article Title: Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fisher Scientific , 12027.

    Article Title: RNA methylation controls stress granule formation and function during erythropoiesis
    Article Snippet: End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25 0 C for 45 min. End-repaired DNA was purified using AMPure XP Beads (1.8X of the reaction volume) (Agencourt AMPure XP – PCR purification Beads, BeckmanCoulter, A63881) and separating beads using DynaMag-96 Side Skirted Magnet (Life Technologies, 12027).

    Polymerase Chain Reaction:

    Article Title: Immune correlates of HIV-1 reservoir cell decline in early-treated infants
    Article Snippet: DynaMag-96 Side Skirted Magnet , ThermoFisher Scientific , 12027.

    Article Title: Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
    Article Snippet: Invitrogen DynaMag-96 side skirted magnet , Thermo Fisher Scientific , Cat# 12027.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: Animals Name Company Catalog Number Adiponectin-Cre mouse The Jackson Laboratory 28020 NuTRAP mouse The Jackson Laboratory 29899 Reagents & Materials Name Company Catalog Number 1.5 mL DNA-LoBind tubes Eppendorf 86–923 100 μm cell strainer Falcon 352–360 15 mL tubes VWR 525–1071 2X TD buffer Illumina 15027866 384-well PCR plate Applied biosystem 4483285 40 μm cell strainer Falcon 352–340 50 mL tubes VWR 525–1077 AMPure XP reagent (SPRI beads) Beckman Coulter A63881 Bioanalyzer High Sensitivity DNA kit Agilent Technologies 5067–4626 Clear adhesive film Applied biosystem 4306311 DNase/RNase-free distilled water Invitrogen 10977015 Dounce tissue grinder DWK Life Sciences 357542 DTT Sigma D9779 DynaMag-96 side skirted magnet Thermo Fishers 12027 FACS tubes Falcon 28719128 HEPES Boston BioProducts BBH-75 Hoechst 33342 Invitrogen 2134015 KCl (2 M) Boston BioProducts MT-252 Magnetic separation rack for PCR 8-tube strips EpiCypher 10–0008 MgCl 2 (1 M) Boston BioProducts MT-200 MinElute PCR purification kit Qiagen 28004 NEBNext High-Fidelity 2X PCR master mix BioLabs M0541S NP40 Thermo Fishers 28324 PCR 8-tube strip USA scientific 1402–4708 Protease inhibitor cocktail (100X) Thermo Fishers 78439 Qubit dsDNA HS assay kit Invitrogen Q32851 Sucrose Sigma S0389–1KG SYBR Green I (10,000X) Invitrogen S7563 TDE I enzyme Illumina 15027865 Instruments Name Company Catalog Number Flow cytometer BD Biosciences FACSAria TM Fusion Qubit fluorometer Invitrogen Q33226 Real-Time PCR system Thermo Fishers QuantStudio 5 Open in a separate window Table of Materials.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fishers , 12027.

    Article Title: Innate acting memory Th1 cells modulate heterologous diseases
    Article Snippet: Briefly, Agencourt RNAClean XP paramagnetic beads (Beckman Coulter) were used in combination with a DynaMag-96 side skirted magnet (Thermo Fisher). cDNA was generated with the SuperScript II Reverse Transcriptase Kit (Thermo Fisher) and amplified with HiFi HotStart PCR Mix (KAPA Biosystems).

    Article Title: Product and methods useful for modulating and evaluating immune responses
    Article Snippet: The lysate plate was transferred to a magnet (DynaMag-96 Side Skirted Magnet, Life Technologies), the supernatant was removed, and the beads were washed three times in 100 μL of 80% ethanol, with care being taken to avoid loss of beads during the washes.

    Article Title: Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fisher Scientific , 12027.

    Article Title: RNA methylation controls stress granule formation and function during erythropoiesis
    Article Snippet: End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25 0 C for 45 min. End-repaired DNA was purified using AMPure XP Beads (1.8X of the reaction volume) (Agencourt AMPure XP – PCR purification Beads, BeckmanCoulter, A63881) and separating beads using DynaMag-96 Side Skirted Magnet (Life Technologies, 12027).

    Generated:

    Article Title: Immune correlates of HIV-1 reservoir cell decline in early-treated infants
    Article Snippet: DynaMag-96 Side Skirted Magnet , ThermoFisher Scientific , 12027.

    Article Title: Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
    Article Snippet: Invitrogen DynaMag-96 side skirted magnet , Thermo Fisher Scientific , Cat# 12027.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: Animals Name Company Catalog Number Adiponectin-Cre mouse The Jackson Laboratory 28020 NuTRAP mouse The Jackson Laboratory 29899 Reagents & Materials Name Company Catalog Number 1.5 mL DNA-LoBind tubes Eppendorf 86–923 100 μm cell strainer Falcon 352–360 15 mL tubes VWR 525–1071 2X TD buffer Illumina 15027866 384-well PCR plate Applied biosystem 4483285 40 μm cell strainer Falcon 352–340 50 mL tubes VWR 525–1077 AMPure XP reagent (SPRI beads) Beckman Coulter A63881 Bioanalyzer High Sensitivity DNA kit Agilent Technologies 5067–4626 Clear adhesive film Applied biosystem 4306311 DNase/RNase-free distilled water Invitrogen 10977015 Dounce tissue grinder DWK Life Sciences 357542 DTT Sigma D9779 DynaMag-96 side skirted magnet Thermo Fishers 12027 FACS tubes Falcon 28719128 HEPES Boston BioProducts BBH-75 Hoechst 33342 Invitrogen 2134015 KCl (2 M) Boston BioProducts MT-252 Magnetic separation rack for PCR 8-tube strips EpiCypher 10–0008 MgCl 2 (1 M) Boston BioProducts MT-200 MinElute PCR purification kit Qiagen 28004 NEBNext High-Fidelity 2X PCR master mix BioLabs M0541S NP40 Thermo Fishers 28324 PCR 8-tube strip USA scientific 1402–4708 Protease inhibitor cocktail (100X) Thermo Fishers 78439 Qubit dsDNA HS assay kit Invitrogen Q32851 Sucrose Sigma S0389–1KG SYBR Green I (10,000X) Invitrogen S7563 TDE I enzyme Illumina 15027865 Instruments Name Company Catalog Number Flow cytometer BD Biosciences FACSAria TM Fusion Qubit fluorometer Invitrogen Q33226 Real-Time PCR system Thermo Fishers QuantStudio 5 Open in a separate window Table of Materials.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fishers , 12027.

    Article Title: Innate acting memory Th1 cells modulate heterologous diseases
    Article Snippet: Briefly, Agencourt RNAClean XP paramagnetic beads (Beckman Coulter) were used in combination with a DynaMag-96 side skirted magnet (Thermo Fisher). cDNA was generated with the SuperScript II Reverse Transcriptase Kit (Thermo Fisher) and amplified with HiFi HotStart PCR Mix (KAPA Biosystems).

    Article Title: Product and methods useful for modulating and evaluating immune responses
    Article Snippet: The lysate plate was transferred to a magnet (DynaMag-96 Side Skirted Magnet, Life Technologies), the supernatant was removed, and the beads were washed three times in 100 μL of 80% ethanol, with care being taken to avoid loss of beads during the washes.

    Article Title: Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fisher Scientific , 12027.

    Article Title: RNA methylation controls stress granule formation and function during erythropoiesis
    Article Snippet: End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25 0 C for 45 min. End-repaired DNA was purified using AMPure XP Beads (1.8X of the reaction volume) (Agencourt AMPure XP – PCR purification Beads, BeckmanCoulter, A63881) and separating beads using DynaMag-96 Side Skirted Magnet (Life Technologies, 12027).

    Amplification:

    Article Title: Immune correlates of HIV-1 reservoir cell decline in early-treated infants
    Article Snippet: DynaMag-96 Side Skirted Magnet , ThermoFisher Scientific , 12027.

    Article Title: Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
    Article Snippet: Invitrogen DynaMag-96 side skirted magnet , Thermo Fisher Scientific , Cat# 12027.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: Animals Name Company Catalog Number Adiponectin-Cre mouse The Jackson Laboratory 28020 NuTRAP mouse The Jackson Laboratory 29899 Reagents & Materials Name Company Catalog Number 1.5 mL DNA-LoBind tubes Eppendorf 86–923 100 μm cell strainer Falcon 352–360 15 mL tubes VWR 525–1071 2X TD buffer Illumina 15027866 384-well PCR plate Applied biosystem 4483285 40 μm cell strainer Falcon 352–340 50 mL tubes VWR 525–1077 AMPure XP reagent (SPRI beads) Beckman Coulter A63881 Bioanalyzer High Sensitivity DNA kit Agilent Technologies 5067–4626 Clear adhesive film Applied biosystem 4306311 DNase/RNase-free distilled water Invitrogen 10977015 Dounce tissue grinder DWK Life Sciences 357542 DTT Sigma D9779 DynaMag-96 side skirted magnet Thermo Fishers 12027 FACS tubes Falcon 28719128 HEPES Boston BioProducts BBH-75 Hoechst 33342 Invitrogen 2134015 KCl (2 M) Boston BioProducts MT-252 Magnetic separation rack for PCR 8-tube strips EpiCypher 10–0008 MgCl 2 (1 M) Boston BioProducts MT-200 MinElute PCR purification kit Qiagen 28004 NEBNext High-Fidelity 2X PCR master mix BioLabs M0541S NP40 Thermo Fishers 28324 PCR 8-tube strip USA scientific 1402–4708 Protease inhibitor cocktail (100X) Thermo Fishers 78439 Qubit dsDNA HS assay kit Invitrogen Q32851 Sucrose Sigma S0389–1KG SYBR Green I (10,000X) Invitrogen S7563 TDE I enzyme Illumina 15027865 Instruments Name Company Catalog Number Flow cytometer BD Biosciences FACSAria TM Fusion Qubit fluorometer Invitrogen Q33226 Real-Time PCR system Thermo Fishers QuantStudio 5 Open in a separate window Table of Materials.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fishers , 12027.

    Article Title: Innate acting memory Th1 cells modulate heterologous diseases
    Article Snippet: Briefly, Agencourt RNAClean XP paramagnetic beads (Beckman Coulter) were used in combination with a DynaMag-96 side skirted magnet (Thermo Fisher). cDNA was generated with the SuperScript II Reverse Transcriptase Kit (Thermo Fisher) and amplified with HiFi HotStart PCR Mix (KAPA Biosystems).

    Article Title: Product and methods useful for modulating and evaluating immune responses
    Article Snippet: The lysate plate was transferred to a magnet (DynaMag-96 Side Skirted Magnet, Life Technologies), the supernatant was removed, and the beads were washed three times in 100 μL of 80% ethanol, with care being taken to avoid loss of beads during the washes.

    Article Title: Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fisher Scientific , 12027.

    Article Title: RNA methylation controls stress granule formation and function during erythropoiesis
    Article Snippet: End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25 0 C for 45 min. End-repaired DNA was purified using AMPure XP Beads (1.8X of the reaction volume) (Agencourt AMPure XP – PCR purification Beads, BeckmanCoulter, A63881) and separating beads using DynaMag-96 Side Skirted Magnet (Life Technologies, 12027).

    FACS:

    Article Title: Immune correlates of HIV-1 reservoir cell decline in early-treated infants
    Article Snippet: DynaMag-96 Side Skirted Magnet , ThermoFisher Scientific , 12027.

    Article Title: Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
    Article Snippet: Invitrogen DynaMag-96 side skirted magnet , Thermo Fisher Scientific , Cat# 12027.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: Animals Name Company Catalog Number Adiponectin-Cre mouse The Jackson Laboratory 28020 NuTRAP mouse The Jackson Laboratory 29899 Reagents & Materials Name Company Catalog Number 1.5 mL DNA-LoBind tubes Eppendorf 86–923 100 μm cell strainer Falcon 352–360 15 mL tubes VWR 525–1071 2X TD buffer Illumina 15027866 384-well PCR plate Applied biosystem 4483285 40 μm cell strainer Falcon 352–340 50 mL tubes VWR 525–1077 AMPure XP reagent (SPRI beads) Beckman Coulter A63881 Bioanalyzer High Sensitivity DNA kit Agilent Technologies 5067–4626 Clear adhesive film Applied biosystem 4306311 DNase/RNase-free distilled water Invitrogen 10977015 Dounce tissue grinder DWK Life Sciences 357542 DTT Sigma D9779 DynaMag-96 side skirted magnet Thermo Fishers 12027 FACS tubes Falcon 28719128 HEPES Boston BioProducts BBH-75 Hoechst 33342 Invitrogen 2134015 KCl (2 M) Boston BioProducts MT-252 Magnetic separation rack for PCR 8-tube strips EpiCypher 10–0008 MgCl 2 (1 M) Boston BioProducts MT-200 MinElute PCR purification kit Qiagen 28004 NEBNext High-Fidelity 2X PCR master mix BioLabs M0541S NP40 Thermo Fishers 28324 PCR 8-tube strip USA scientific 1402–4708 Protease inhibitor cocktail (100X) Thermo Fishers 78439 Qubit dsDNA HS assay kit Invitrogen Q32851 Sucrose Sigma S0389–1KG SYBR Green I (10,000X) Invitrogen S7563 TDE I enzyme Illumina 15027865 Instruments Name Company Catalog Number Flow cytometer BD Biosciences FACSAria TM Fusion Qubit fluorometer Invitrogen Q33226 Real-Time PCR system Thermo Fishers QuantStudio 5 Open in a separate window Table of Materials.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fishers , 12027.

    Article Title: Innate acting memory Th1 cells modulate heterologous diseases
    Article Snippet: Briefly, Agencourt RNAClean XP paramagnetic beads (Beckman Coulter) were used in combination with a DynaMag-96 side skirted magnet (Thermo Fisher). cDNA was generated with the SuperScript II Reverse Transcriptase Kit (Thermo Fisher) and amplified with HiFi HotStart PCR Mix (KAPA Biosystems).

    Article Title: Product and methods useful for modulating and evaluating immune responses
    Article Snippet: The lysate plate was transferred to a magnet (DynaMag-96 Side Skirted Magnet, Life Technologies), the supernatant was removed, and the beads were washed three times in 100 μL of 80% ethanol, with care being taken to avoid loss of beads during the washes.

    Article Title: Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fisher Scientific , 12027.

    Article Title: RNA methylation controls stress granule formation and function during erythropoiesis
    Article Snippet: End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25 0 C for 45 min. End-repaired DNA was purified using AMPure XP Beads (1.8X of the reaction volume) (Agencourt AMPure XP – PCR purification Beads, BeckmanCoulter, A63881) and separating beads using DynaMag-96 Side Skirted Magnet (Life Technologies, 12027).

    Stripping Membranes:

    Article Title: Immune correlates of HIV-1 reservoir cell decline in early-treated infants
    Article Snippet: DynaMag-96 Side Skirted Magnet , ThermoFisher Scientific , 12027.

    Article Title: Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
    Article Snippet: Invitrogen DynaMag-96 side skirted magnet , Thermo Fisher Scientific , Cat# 12027.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: Animals Name Company Catalog Number Adiponectin-Cre mouse The Jackson Laboratory 28020 NuTRAP mouse The Jackson Laboratory 29899 Reagents & Materials Name Company Catalog Number 1.5 mL DNA-LoBind tubes Eppendorf 86–923 100 μm cell strainer Falcon 352–360 15 mL tubes VWR 525–1071 2X TD buffer Illumina 15027866 384-well PCR plate Applied biosystem 4483285 40 μm cell strainer Falcon 352–340 50 mL tubes VWR 525–1077 AMPure XP reagent (SPRI beads) Beckman Coulter A63881 Bioanalyzer High Sensitivity DNA kit Agilent Technologies 5067–4626 Clear adhesive film Applied biosystem 4306311 DNase/RNase-free distilled water Invitrogen 10977015 Dounce tissue grinder DWK Life Sciences 357542 DTT Sigma D9779 DynaMag-96 side skirted magnet Thermo Fishers 12027 FACS tubes Falcon 28719128 HEPES Boston BioProducts BBH-75 Hoechst 33342 Invitrogen 2134015 KCl (2 M) Boston BioProducts MT-252 Magnetic separation rack for PCR 8-tube strips EpiCypher 10–0008 MgCl 2 (1 M) Boston BioProducts MT-200 MinElute PCR purification kit Qiagen 28004 NEBNext High-Fidelity 2X PCR master mix BioLabs M0541S NP40 Thermo Fishers 28324 PCR 8-tube strip USA scientific 1402–4708 Protease inhibitor cocktail (100X) Thermo Fishers 78439 Qubit dsDNA HS assay kit Invitrogen Q32851 Sucrose Sigma S0389–1KG SYBR Green I (10,000X) Invitrogen S7563 TDE I enzyme Illumina 15027865 Instruments Name Company Catalog Number Flow cytometer BD Biosciences FACSAria TM Fusion Qubit fluorometer Invitrogen Q33226 Real-Time PCR system Thermo Fishers QuantStudio 5 Open in a separate window Table of Materials.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fishers , 12027.

    Article Title: Innate acting memory Th1 cells modulate heterologous diseases
    Article Snippet: Briefly, Agencourt RNAClean XP paramagnetic beads (Beckman Coulter) were used in combination with a DynaMag-96 side skirted magnet (Thermo Fisher). cDNA was generated with the SuperScript II Reverse Transcriptase Kit (Thermo Fisher) and amplified with HiFi HotStart PCR Mix (KAPA Biosystems).

    Article Title: Product and methods useful for modulating and evaluating immune responses
    Article Snippet: The lysate plate was transferred to a magnet (DynaMag-96 Side Skirted Magnet, Life Technologies), the supernatant was removed, and the beads were washed three times in 100 μL of 80% ethanol, with care being taken to avoid loss of beads during the washes.

    Article Title: Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fisher Scientific , 12027.

    Article Title: RNA methylation controls stress granule formation and function during erythropoiesis
    Article Snippet: End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25 0 C for 45 min. End-repaired DNA was purified using AMPure XP Beads (1.8X of the reaction volume) (Agencourt AMPure XP – PCR purification Beads, BeckmanCoulter, A63881) and separating beads using DynaMag-96 Side Skirted Magnet (Life Technologies, 12027).

    Protease Inhibitor:

    Article Title: Immune correlates of HIV-1 reservoir cell decline in early-treated infants
    Article Snippet: DynaMag-96 Side Skirted Magnet , ThermoFisher Scientific , 12027.

    Article Title: Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
    Article Snippet: Invitrogen DynaMag-96 side skirted magnet , Thermo Fisher Scientific , Cat# 12027.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: Animals Name Company Catalog Number Adiponectin-Cre mouse The Jackson Laboratory 28020 NuTRAP mouse The Jackson Laboratory 29899 Reagents & Materials Name Company Catalog Number 1.5 mL DNA-LoBind tubes Eppendorf 86–923 100 μm cell strainer Falcon 352–360 15 mL tubes VWR 525–1071 2X TD buffer Illumina 15027866 384-well PCR plate Applied biosystem 4483285 40 μm cell strainer Falcon 352–340 50 mL tubes VWR 525–1077 AMPure XP reagent (SPRI beads) Beckman Coulter A63881 Bioanalyzer High Sensitivity DNA kit Agilent Technologies 5067–4626 Clear adhesive film Applied biosystem 4306311 DNase/RNase-free distilled water Invitrogen 10977015 Dounce tissue grinder DWK Life Sciences 357542 DTT Sigma D9779 DynaMag-96 side skirted magnet Thermo Fishers 12027 FACS tubes Falcon 28719128 HEPES Boston BioProducts BBH-75 Hoechst 33342 Invitrogen 2134015 KCl (2 M) Boston BioProducts MT-252 Magnetic separation rack for PCR 8-tube strips EpiCypher 10–0008 MgCl 2 (1 M) Boston BioProducts MT-200 MinElute PCR purification kit Qiagen 28004 NEBNext High-Fidelity 2X PCR master mix BioLabs M0541S NP40 Thermo Fishers 28324 PCR 8-tube strip USA scientific 1402–4708 Protease inhibitor cocktail (100X) Thermo Fishers 78439 Qubit dsDNA HS assay kit Invitrogen Q32851 Sucrose Sigma S0389–1KG SYBR Green I (10,000X) Invitrogen S7563 TDE I enzyme Illumina 15027865 Instruments Name Company Catalog Number Flow cytometer BD Biosciences FACSAria TM Fusion Qubit fluorometer Invitrogen Q33226 Real-Time PCR system Thermo Fishers QuantStudio 5 Open in a separate window Table of Materials.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fishers , 12027.

    Article Title: Innate acting memory Th1 cells modulate heterologous diseases
    Article Snippet: Briefly, Agencourt RNAClean XP paramagnetic beads (Beckman Coulter) were used in combination with a DynaMag-96 side skirted magnet (Thermo Fisher). cDNA was generated with the SuperScript II Reverse Transcriptase Kit (Thermo Fisher) and amplified with HiFi HotStart PCR Mix (KAPA Biosystems).

    Article Title: Product and methods useful for modulating and evaluating immune responses
    Article Snippet: The lysate plate was transferred to a magnet (DynaMag-96 Side Skirted Magnet, Life Technologies), the supernatant was removed, and the beads were washed three times in 100 μL of 80% ethanol, with care being taken to avoid loss of beads during the washes.

    Article Title: Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fisher Scientific , 12027.

    Article Title: RNA methylation controls stress granule formation and function during erythropoiesis
    Article Snippet: End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25 0 C for 45 min. End-repaired DNA was purified using AMPure XP Beads (1.8X of the reaction volume) (Agencourt AMPure XP – PCR purification Beads, BeckmanCoulter, A63881) and separating beads using DynaMag-96 Side Skirted Magnet (Life Technologies, 12027).

    SYBR Green Assay:

    Article Title: Immune correlates of HIV-1 reservoir cell decline in early-treated infants
    Article Snippet: DynaMag-96 Side Skirted Magnet , ThermoFisher Scientific , 12027.

    Article Title: Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
    Article Snippet: Invitrogen DynaMag-96 side skirted magnet , Thermo Fisher Scientific , Cat# 12027.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: Animals Name Company Catalog Number Adiponectin-Cre mouse The Jackson Laboratory 28020 NuTRAP mouse The Jackson Laboratory 29899 Reagents & Materials Name Company Catalog Number 1.5 mL DNA-LoBind tubes Eppendorf 86–923 100 μm cell strainer Falcon 352–360 15 mL tubes VWR 525–1071 2X TD buffer Illumina 15027866 384-well PCR plate Applied biosystem 4483285 40 μm cell strainer Falcon 352–340 50 mL tubes VWR 525–1077 AMPure XP reagent (SPRI beads) Beckman Coulter A63881 Bioanalyzer High Sensitivity DNA kit Agilent Technologies 5067–4626 Clear adhesive film Applied biosystem 4306311 DNase/RNase-free distilled water Invitrogen 10977015 Dounce tissue grinder DWK Life Sciences 357542 DTT Sigma D9779 DynaMag-96 side skirted magnet Thermo Fishers 12027 FACS tubes Falcon 28719128 HEPES Boston BioProducts BBH-75 Hoechst 33342 Invitrogen 2134015 KCl (2 M) Boston BioProducts MT-252 Magnetic separation rack for PCR 8-tube strips EpiCypher 10–0008 MgCl 2 (1 M) Boston BioProducts MT-200 MinElute PCR purification kit Qiagen 28004 NEBNext High-Fidelity 2X PCR master mix BioLabs M0541S NP40 Thermo Fishers 28324 PCR 8-tube strip USA scientific 1402–4708 Protease inhibitor cocktail (100X) Thermo Fishers 78439 Qubit dsDNA HS assay kit Invitrogen Q32851 Sucrose Sigma S0389–1KG SYBR Green I (10,000X) Invitrogen S7563 TDE I enzyme Illumina 15027865 Instruments Name Company Catalog Number Flow cytometer BD Biosciences FACSAria TM Fusion Qubit fluorometer Invitrogen Q33226 Real-Time PCR system Thermo Fishers QuantStudio 5 Open in a separate window Table of Materials.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fishers , 12027.

    Article Title: Innate acting memory Th1 cells modulate heterologous diseases
    Article Snippet: Briefly, Agencourt RNAClean XP paramagnetic beads (Beckman Coulter) were used in combination with a DynaMag-96 side skirted magnet (Thermo Fisher). cDNA was generated with the SuperScript II Reverse Transcriptase Kit (Thermo Fisher) and amplified with HiFi HotStart PCR Mix (KAPA Biosystems).

    Article Title: Product and methods useful for modulating and evaluating immune responses
    Article Snippet: The lysate plate was transferred to a magnet (DynaMag-96 Side Skirted Magnet, Life Technologies), the supernatant was removed, and the beads were washed three times in 100 μL of 80% ethanol, with care being taken to avoid loss of beads during the washes.

    Article Title: Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fisher Scientific , 12027.

    Article Title: RNA methylation controls stress granule formation and function during erythropoiesis
    Article Snippet: End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25 0 C for 45 min. End-repaired DNA was purified using AMPure XP Beads (1.8X of the reaction volume) (Agencourt AMPure XP – PCR purification Beads, BeckmanCoulter, A63881) and separating beads using DynaMag-96 Side Skirted Magnet (Life Technologies, 12027).

    Flow Cytometry:

    Article Title: Immune correlates of HIV-1 reservoir cell decline in early-treated infants
    Article Snippet: DynaMag-96 Side Skirted Magnet , ThermoFisher Scientific , 12027.

    Article Title: Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
    Article Snippet: Invitrogen DynaMag-96 side skirted magnet , Thermo Fisher Scientific , Cat# 12027.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: Animals Name Company Catalog Number Adiponectin-Cre mouse The Jackson Laboratory 28020 NuTRAP mouse The Jackson Laboratory 29899 Reagents & Materials Name Company Catalog Number 1.5 mL DNA-LoBind tubes Eppendorf 86–923 100 μm cell strainer Falcon 352–360 15 mL tubes VWR 525–1071 2X TD buffer Illumina 15027866 384-well PCR plate Applied biosystem 4483285 40 μm cell strainer Falcon 352–340 50 mL tubes VWR 525–1077 AMPure XP reagent (SPRI beads) Beckman Coulter A63881 Bioanalyzer High Sensitivity DNA kit Agilent Technologies 5067–4626 Clear adhesive film Applied biosystem 4306311 DNase/RNase-free distilled water Invitrogen 10977015 Dounce tissue grinder DWK Life Sciences 357542 DTT Sigma D9779 DynaMag-96 side skirted magnet Thermo Fishers 12027 FACS tubes Falcon 28719128 HEPES Boston BioProducts BBH-75 Hoechst 33342 Invitrogen 2134015 KCl (2 M) Boston BioProducts MT-252 Magnetic separation rack for PCR 8-tube strips EpiCypher 10–0008 MgCl 2 (1 M) Boston BioProducts MT-200 MinElute PCR purification kit Qiagen 28004 NEBNext High-Fidelity 2X PCR master mix BioLabs M0541S NP40 Thermo Fishers 28324 PCR 8-tube strip USA scientific 1402–4708 Protease inhibitor cocktail (100X) Thermo Fishers 78439 Qubit dsDNA HS assay kit Invitrogen Q32851 Sucrose Sigma S0389–1KG SYBR Green I (10,000X) Invitrogen S7563 TDE I enzyme Illumina 15027865 Instruments Name Company Catalog Number Flow cytometer BD Biosciences FACSAria TM Fusion Qubit fluorometer Invitrogen Q33226 Real-Time PCR system Thermo Fishers QuantStudio 5 Open in a separate window Table of Materials.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fishers , 12027.

    Article Title: Innate acting memory Th1 cells modulate heterologous diseases
    Article Snippet: Briefly, Agencourt RNAClean XP paramagnetic beads (Beckman Coulter) were used in combination with a DynaMag-96 side skirted magnet (Thermo Fisher). cDNA was generated with the SuperScript II Reverse Transcriptase Kit (Thermo Fisher) and amplified with HiFi HotStart PCR Mix (KAPA Biosystems).

    Article Title: Product and methods useful for modulating and evaluating immune responses
    Article Snippet: The lysate plate was transferred to a magnet (DynaMag-96 Side Skirted Magnet, Life Technologies), the supernatant was removed, and the beads were washed three times in 100 μL of 80% ethanol, with care being taken to avoid loss of beads during the washes.

    Article Title: Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fisher Scientific , 12027.

    Article Title: RNA methylation controls stress granule formation and function during erythropoiesis
    Article Snippet: End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25 0 C for 45 min. End-repaired DNA was purified using AMPure XP Beads (1.8X of the reaction volume) (Agencourt AMPure XP – PCR purification Beads, BeckmanCoulter, A63881) and separating beads using DynaMag-96 Side Skirted Magnet (Life Technologies, 12027).

    Real-time Polymerase Chain Reaction:

    Article Title: Immune correlates of HIV-1 reservoir cell decline in early-treated infants
    Article Snippet: DynaMag-96 Side Skirted Magnet , ThermoFisher Scientific , 12027.

    Article Title: Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
    Article Snippet: Invitrogen DynaMag-96 side skirted magnet , Thermo Fisher Scientific , Cat# 12027.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: Animals Name Company Catalog Number Adiponectin-Cre mouse The Jackson Laboratory 28020 NuTRAP mouse The Jackson Laboratory 29899 Reagents & Materials Name Company Catalog Number 1.5 mL DNA-LoBind tubes Eppendorf 86–923 100 μm cell strainer Falcon 352–360 15 mL tubes VWR 525–1071 2X TD buffer Illumina 15027866 384-well PCR plate Applied biosystem 4483285 40 μm cell strainer Falcon 352–340 50 mL tubes VWR 525–1077 AMPure XP reagent (SPRI beads) Beckman Coulter A63881 Bioanalyzer High Sensitivity DNA kit Agilent Technologies 5067–4626 Clear adhesive film Applied biosystem 4306311 DNase/RNase-free distilled water Invitrogen 10977015 Dounce tissue grinder DWK Life Sciences 357542 DTT Sigma D9779 DynaMag-96 side skirted magnet Thermo Fishers 12027 FACS tubes Falcon 28719128 HEPES Boston BioProducts BBH-75 Hoechst 33342 Invitrogen 2134015 KCl (2 M) Boston BioProducts MT-252 Magnetic separation rack for PCR 8-tube strips EpiCypher 10–0008 MgCl 2 (1 M) Boston BioProducts MT-200 MinElute PCR purification kit Qiagen 28004 NEBNext High-Fidelity 2X PCR master mix BioLabs M0541S NP40 Thermo Fishers 28324 PCR 8-tube strip USA scientific 1402–4708 Protease inhibitor cocktail (100X) Thermo Fishers 78439 Qubit dsDNA HS assay kit Invitrogen Q32851 Sucrose Sigma S0389–1KG SYBR Green I (10,000X) Invitrogen S7563 TDE I enzyme Illumina 15027865 Instruments Name Company Catalog Number Flow cytometer BD Biosciences FACSAria TM Fusion Qubit fluorometer Invitrogen Q33226 Real-Time PCR system Thermo Fishers QuantStudio 5 Open in a separate window Table of Materials.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fishers , 12027.

    Article Title: Innate acting memory Th1 cells modulate heterologous diseases
    Article Snippet: Briefly, Agencourt RNAClean XP paramagnetic beads (Beckman Coulter) were used in combination with a DynaMag-96 side skirted magnet (Thermo Fisher). cDNA was generated with the SuperScript II Reverse Transcriptase Kit (Thermo Fisher) and amplified with HiFi HotStart PCR Mix (KAPA Biosystems).

    Article Title: Product and methods useful for modulating and evaluating immune responses
    Article Snippet: The lysate plate was transferred to a magnet (DynaMag-96 Side Skirted Magnet, Life Technologies), the supernatant was removed, and the beads were washed three times in 100 μL of 80% ethanol, with care being taken to avoid loss of beads during the washes.

    Article Title: Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fisher Scientific , 12027.

    Article Title: RNA methylation controls stress granule formation and function during erythropoiesis
    Article Snippet: End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25 0 C for 45 min. End-repaired DNA was purified using AMPure XP Beads (1.8X of the reaction volume) (Agencourt AMPure XP – PCR purification Beads, BeckmanCoulter, A63881) and separating beads using DynaMag-96 Side Skirted Magnet (Life Technologies, 12027).

    Saline:

    Article Title: Immune correlates of HIV-1 reservoir cell decline in early-treated infants
    Article Snippet: DynaMag-96 Side Skirted Magnet , ThermoFisher Scientific , 12027.

    Article Title: Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
    Article Snippet: Invitrogen DynaMag-96 side skirted magnet , Thermo Fisher Scientific , Cat# 12027.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: Animals Name Company Catalog Number Adiponectin-Cre mouse The Jackson Laboratory 28020 NuTRAP mouse The Jackson Laboratory 29899 Reagents & Materials Name Company Catalog Number 1.5 mL DNA-LoBind tubes Eppendorf 86–923 100 μm cell strainer Falcon 352–360 15 mL tubes VWR 525–1071 2X TD buffer Illumina 15027866 384-well PCR plate Applied biosystem 4483285 40 μm cell strainer Falcon 352–340 50 mL tubes VWR 525–1077 AMPure XP reagent (SPRI beads) Beckman Coulter A63881 Bioanalyzer High Sensitivity DNA kit Agilent Technologies 5067–4626 Clear adhesive film Applied biosystem 4306311 DNase/RNase-free distilled water Invitrogen 10977015 Dounce tissue grinder DWK Life Sciences 357542 DTT Sigma D9779 DynaMag-96 side skirted magnet Thermo Fishers 12027 FACS tubes Falcon 28719128 HEPES Boston BioProducts BBH-75 Hoechst 33342 Invitrogen 2134015 KCl (2 M) Boston BioProducts MT-252 Magnetic separation rack for PCR 8-tube strips EpiCypher 10–0008 MgCl 2 (1 M) Boston BioProducts MT-200 MinElute PCR purification kit Qiagen 28004 NEBNext High-Fidelity 2X PCR master mix BioLabs M0541S NP40 Thermo Fishers 28324 PCR 8-tube strip USA scientific 1402–4708 Protease inhibitor cocktail (100X) Thermo Fishers 78439 Qubit dsDNA HS assay kit Invitrogen Q32851 Sucrose Sigma S0389–1KG SYBR Green I (10,000X) Invitrogen S7563 TDE I enzyme Illumina 15027865 Instruments Name Company Catalog Number Flow cytometer BD Biosciences FACSAria TM Fusion Qubit fluorometer Invitrogen Q33226 Real-Time PCR system Thermo Fishers QuantStudio 5 Open in a separate window Table of Materials.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fishers , 12027.

    Article Title: Innate acting memory Th1 cells modulate heterologous diseases
    Article Snippet: Briefly, Agencourt RNAClean XP paramagnetic beads (Beckman Coulter) were used in combination with a DynaMag-96 side skirted magnet (Thermo Fisher). cDNA was generated with the SuperScript II Reverse Transcriptase Kit (Thermo Fisher) and amplified with HiFi HotStart PCR Mix (KAPA Biosystems).

    Article Title: Product and methods useful for modulating and evaluating immune responses
    Article Snippet: The lysate plate was transferred to a magnet (DynaMag-96 Side Skirted Magnet, Life Technologies), the supernatant was removed, and the beads were washed three times in 100 μL of 80% ethanol, with care being taken to avoid loss of beads during the washes.

    Article Title: Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fisher Scientific , 12027.

    Article Title: RNA methylation controls stress granule formation and function during erythropoiesis
    Article Snippet: End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25 0 C for 45 min. End-repaired DNA was purified using AMPure XP Beads (1.8X of the reaction volume) (Agencourt AMPure XP – PCR purification Beads, BeckmanCoulter, A63881) and separating beads using DynaMag-96 Side Skirted Magnet (Life Technologies, 12027).

    Sensitive Assay:

    Article Title: Immune correlates of HIV-1 reservoir cell decline in early-treated infants
    Article Snippet: DynaMag-96 Side Skirted Magnet , ThermoFisher Scientific , 12027.

    Article Title: Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
    Article Snippet: Invitrogen DynaMag-96 side skirted magnet , Thermo Fisher Scientific , Cat# 12027.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: Animals Name Company Catalog Number Adiponectin-Cre mouse The Jackson Laboratory 28020 NuTRAP mouse The Jackson Laboratory 29899 Reagents & Materials Name Company Catalog Number 1.5 mL DNA-LoBind tubes Eppendorf 86–923 100 μm cell strainer Falcon 352–360 15 mL tubes VWR 525–1071 2X TD buffer Illumina 15027866 384-well PCR plate Applied biosystem 4483285 40 μm cell strainer Falcon 352–340 50 mL tubes VWR 525–1077 AMPure XP reagent (SPRI beads) Beckman Coulter A63881 Bioanalyzer High Sensitivity DNA kit Agilent Technologies 5067–4626 Clear adhesive film Applied biosystem 4306311 DNase/RNase-free distilled water Invitrogen 10977015 Dounce tissue grinder DWK Life Sciences 357542 DTT Sigma D9779 DynaMag-96 side skirted magnet Thermo Fishers 12027 FACS tubes Falcon 28719128 HEPES Boston BioProducts BBH-75 Hoechst 33342 Invitrogen 2134015 KCl (2 M) Boston BioProducts MT-252 Magnetic separation rack for PCR 8-tube strips EpiCypher 10–0008 MgCl 2 (1 M) Boston BioProducts MT-200 MinElute PCR purification kit Qiagen 28004 NEBNext High-Fidelity 2X PCR master mix BioLabs M0541S NP40 Thermo Fishers 28324 PCR 8-tube strip USA scientific 1402–4708 Protease inhibitor cocktail (100X) Thermo Fishers 78439 Qubit dsDNA HS assay kit Invitrogen Q32851 Sucrose Sigma S0389–1KG SYBR Green I (10,000X) Invitrogen S7563 TDE I enzyme Illumina 15027865 Instruments Name Company Catalog Number Flow cytometer BD Biosciences FACSAria TM Fusion Qubit fluorometer Invitrogen Q33226 Real-Time PCR system Thermo Fishers QuantStudio 5 Open in a separate window Table of Materials.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fishers , 12027.

    Article Title: Innate acting memory Th1 cells modulate heterologous diseases
    Article Snippet: Briefly, Agencourt RNAClean XP paramagnetic beads (Beckman Coulter) were used in combination with a DynaMag-96 side skirted magnet (Thermo Fisher). cDNA was generated with the SuperScript II Reverse Transcriptase Kit (Thermo Fisher) and amplified with HiFi HotStart PCR Mix (KAPA Biosystems).

    Article Title: Product and methods useful for modulating and evaluating immune responses
    Article Snippet: The lysate plate was transferred to a magnet (DynaMag-96 Side Skirted Magnet, Life Technologies), the supernatant was removed, and the beads were washed three times in 100 μL of 80% ethanol, with care being taken to avoid loss of beads during the washes.

    Article Title: Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fisher Scientific , 12027.

    Article Title: RNA methylation controls stress granule formation and function during erythropoiesis
    Article Snippet: End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25 0 C for 45 min. End-repaired DNA was purified using AMPure XP Beads (1.8X of the reaction volume) (Agencourt AMPure XP – PCR purification Beads, BeckmanCoulter, A63881) and separating beads using DynaMag-96 Side Skirted Magnet (Life Technologies, 12027).

    Transferring:

    Article Title: Immune correlates of HIV-1 reservoir cell decline in early-treated infants
    Article Snippet: DynaMag-96 Side Skirted Magnet , ThermoFisher Scientific , 12027.

    Article Title: Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
    Article Snippet: Invitrogen DynaMag-96 side skirted magnet , Thermo Fisher Scientific , Cat# 12027.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: Animals Name Company Catalog Number Adiponectin-Cre mouse The Jackson Laboratory 28020 NuTRAP mouse The Jackson Laboratory 29899 Reagents & Materials Name Company Catalog Number 1.5 mL DNA-LoBind tubes Eppendorf 86–923 100 μm cell strainer Falcon 352–360 15 mL tubes VWR 525–1071 2X TD buffer Illumina 15027866 384-well PCR plate Applied biosystem 4483285 40 μm cell strainer Falcon 352–340 50 mL tubes VWR 525–1077 AMPure XP reagent (SPRI beads) Beckman Coulter A63881 Bioanalyzer High Sensitivity DNA kit Agilent Technologies 5067–4626 Clear adhesive film Applied biosystem 4306311 DNase/RNase-free distilled water Invitrogen 10977015 Dounce tissue grinder DWK Life Sciences 357542 DTT Sigma D9779 DynaMag-96 side skirted magnet Thermo Fishers 12027 FACS tubes Falcon 28719128 HEPES Boston BioProducts BBH-75 Hoechst 33342 Invitrogen 2134015 KCl (2 M) Boston BioProducts MT-252 Magnetic separation rack for PCR 8-tube strips EpiCypher 10–0008 MgCl 2 (1 M) Boston BioProducts MT-200 MinElute PCR purification kit Qiagen 28004 NEBNext High-Fidelity 2X PCR master mix BioLabs M0541S NP40 Thermo Fishers 28324 PCR 8-tube strip USA scientific 1402–4708 Protease inhibitor cocktail (100X) Thermo Fishers 78439 Qubit dsDNA HS assay kit Invitrogen Q32851 Sucrose Sigma S0389–1KG SYBR Green I (10,000X) Invitrogen S7563 TDE I enzyme Illumina 15027865 Instruments Name Company Catalog Number Flow cytometer BD Biosciences FACSAria TM Fusion Qubit fluorometer Invitrogen Q33226 Real-Time PCR system Thermo Fishers QuantStudio 5 Open in a separate window Table of Materials.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fishers , 12027.

    Article Title: Innate acting memory Th1 cells modulate heterologous diseases
    Article Snippet: Briefly, Agencourt RNAClean XP paramagnetic beads (Beckman Coulter) were used in combination with a DynaMag-96 side skirted magnet (Thermo Fisher). cDNA was generated with the SuperScript II Reverse Transcriptase Kit (Thermo Fisher) and amplified with HiFi HotStart PCR Mix (KAPA Biosystems).

    Article Title: Product and methods useful for modulating and evaluating immune responses
    Article Snippet: The lysate plate was transferred to a magnet (DynaMag-96 Side Skirted Magnet, Life Technologies), the supernatant was removed, and the beads were washed three times in 100 μL of 80% ethanol, with care being taken to avoid loss of beads during the washes.

    Article Title: Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fisher Scientific , 12027.

    Article Title: RNA methylation controls stress granule formation and function during erythropoiesis
    Article Snippet: End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25 0 C for 45 min. End-repaired DNA was purified using AMPure XP Beads (1.8X of the reaction volume) (Agencourt AMPure XP – PCR purification Beads, BeckmanCoulter, A63881) and separating beads using DynaMag-96 Side Skirted Magnet (Life Technologies, 12027).

    Incubation:

    Article Title: Immune correlates of HIV-1 reservoir cell decline in early-treated infants
    Article Snippet: DynaMag-96 Side Skirted Magnet , ThermoFisher Scientific , 12027.

    Article Title: Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
    Article Snippet: Invitrogen DynaMag-96 side skirted magnet , Thermo Fisher Scientific , Cat# 12027.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: Animals Name Company Catalog Number Adiponectin-Cre mouse The Jackson Laboratory 28020 NuTRAP mouse The Jackson Laboratory 29899 Reagents & Materials Name Company Catalog Number 1.5 mL DNA-LoBind tubes Eppendorf 86–923 100 μm cell strainer Falcon 352–360 15 mL tubes VWR 525–1071 2X TD buffer Illumina 15027866 384-well PCR plate Applied biosystem 4483285 40 μm cell strainer Falcon 352–340 50 mL tubes VWR 525–1077 AMPure XP reagent (SPRI beads) Beckman Coulter A63881 Bioanalyzer High Sensitivity DNA kit Agilent Technologies 5067–4626 Clear adhesive film Applied biosystem 4306311 DNase/RNase-free distilled water Invitrogen 10977015 Dounce tissue grinder DWK Life Sciences 357542 DTT Sigma D9779 DynaMag-96 side skirted magnet Thermo Fishers 12027 FACS tubes Falcon 28719128 HEPES Boston BioProducts BBH-75 Hoechst 33342 Invitrogen 2134015 KCl (2 M) Boston BioProducts MT-252 Magnetic separation rack for PCR 8-tube strips EpiCypher 10–0008 MgCl 2 (1 M) Boston BioProducts MT-200 MinElute PCR purification kit Qiagen 28004 NEBNext High-Fidelity 2X PCR master mix BioLabs M0541S NP40 Thermo Fishers 28324 PCR 8-tube strip USA scientific 1402–4708 Protease inhibitor cocktail (100X) Thermo Fishers 78439 Qubit dsDNA HS assay kit Invitrogen Q32851 Sucrose Sigma S0389–1KG SYBR Green I (10,000X) Invitrogen S7563 TDE I enzyme Illumina 15027865 Instruments Name Company Catalog Number Flow cytometer BD Biosciences FACSAria TM Fusion Qubit fluorometer Invitrogen Q33226 Real-Time PCR system Thermo Fishers QuantStudio 5 Open in a separate window Table of Materials.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fishers , 12027.

    Article Title: Innate acting memory Th1 cells modulate heterologous diseases
    Article Snippet: Briefly, Agencourt RNAClean XP paramagnetic beads (Beckman Coulter) were used in combination with a DynaMag-96 side skirted magnet (Thermo Fisher). cDNA was generated with the SuperScript II Reverse Transcriptase Kit (Thermo Fisher) and amplified with HiFi HotStart PCR Mix (KAPA Biosystems).

    Article Title: Product and methods useful for modulating and evaluating immune responses
    Article Snippet: The lysate plate was transferred to a magnet (DynaMag-96 Side Skirted Magnet, Life Technologies), the supernatant was removed, and the beads were washed three times in 100 μL of 80% ethanol, with care being taken to avoid loss of beads during the washes.

    Article Title: Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fisher Scientific , 12027.

    Article Title: RNA methylation controls stress granule formation and function during erythropoiesis
    Article Snippet: End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25 0 C for 45 min. End-repaired DNA was purified using AMPure XP Beads (1.8X of the reaction volume) (Agencourt AMPure XP – PCR purification Beads, BeckmanCoulter, A63881) and separating beads using DynaMag-96 Side Skirted Magnet (Life Technologies, 12027).

    Staining:

    Article Title: Immune correlates of HIV-1 reservoir cell decline in early-treated infants
    Article Snippet: DynaMag-96 Side Skirted Magnet , ThermoFisher Scientific , 12027.

    Article Title: Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
    Article Snippet: Invitrogen DynaMag-96 side skirted magnet , Thermo Fisher Scientific , Cat# 12027.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: Animals Name Company Catalog Number Adiponectin-Cre mouse The Jackson Laboratory 28020 NuTRAP mouse The Jackson Laboratory 29899 Reagents & Materials Name Company Catalog Number 1.5 mL DNA-LoBind tubes Eppendorf 86–923 100 μm cell strainer Falcon 352–360 15 mL tubes VWR 525–1071 2X TD buffer Illumina 15027866 384-well PCR plate Applied biosystem 4483285 40 μm cell strainer Falcon 352–340 50 mL tubes VWR 525–1077 AMPure XP reagent (SPRI beads) Beckman Coulter A63881 Bioanalyzer High Sensitivity DNA kit Agilent Technologies 5067–4626 Clear adhesive film Applied biosystem 4306311 DNase/RNase-free distilled water Invitrogen 10977015 Dounce tissue grinder DWK Life Sciences 357542 DTT Sigma D9779 DynaMag-96 side skirted magnet Thermo Fishers 12027 FACS tubes Falcon 28719128 HEPES Boston BioProducts BBH-75 Hoechst 33342 Invitrogen 2134015 KCl (2 M) Boston BioProducts MT-252 Magnetic separation rack for PCR 8-tube strips EpiCypher 10–0008 MgCl 2 (1 M) Boston BioProducts MT-200 MinElute PCR purification kit Qiagen 28004 NEBNext High-Fidelity 2X PCR master mix BioLabs M0541S NP40 Thermo Fishers 28324 PCR 8-tube strip USA scientific 1402–4708 Protease inhibitor cocktail (100X) Thermo Fishers 78439 Qubit dsDNA HS assay kit Invitrogen Q32851 Sucrose Sigma S0389–1KG SYBR Green I (10,000X) Invitrogen S7563 TDE I enzyme Illumina 15027865 Instruments Name Company Catalog Number Flow cytometer BD Biosciences FACSAria TM Fusion Qubit fluorometer Invitrogen Q33226 Real-Time PCR system Thermo Fishers QuantStudio 5 Open in a separate window Table of Materials.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fishers , 12027.

    Article Title: Innate acting memory Th1 cells modulate heterologous diseases
    Article Snippet: Briefly, Agencourt RNAClean XP paramagnetic beads (Beckman Coulter) were used in combination with a DynaMag-96 side skirted magnet (Thermo Fisher). cDNA was generated with the SuperScript II Reverse Transcriptase Kit (Thermo Fisher) and amplified with HiFi HotStart PCR Mix (KAPA Biosystems).

    Article Title: Product and methods useful for modulating and evaluating immune responses
    Article Snippet: The lysate plate was transferred to a magnet (DynaMag-96 Side Skirted Magnet, Life Technologies), the supernatant was removed, and the beads were washed three times in 100 μL of 80% ethanol, with care being taken to avoid loss of beads during the washes.

    Article Title: Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fisher Scientific , 12027.

    Article Title: RNA methylation controls stress granule formation and function during erythropoiesis
    Article Snippet: End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25 0 C for 45 min. End-repaired DNA was purified using AMPure XP Beads (1.8X of the reaction volume) (Agencourt AMPure XP – PCR purification Beads, BeckmanCoulter, A63881) and separating beads using DynaMag-96 Side Skirted Magnet (Life Technologies, 12027).

    Blocking Assay:

    Article Title: Immune correlates of HIV-1 reservoir cell decline in early-treated infants
    Article Snippet: DynaMag-96 Side Skirted Magnet , ThermoFisher Scientific , 12027.

    Article Title: Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
    Article Snippet: Invitrogen DynaMag-96 side skirted magnet , Thermo Fisher Scientific , Cat# 12027.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: Animals Name Company Catalog Number Adiponectin-Cre mouse The Jackson Laboratory 28020 NuTRAP mouse The Jackson Laboratory 29899 Reagents & Materials Name Company Catalog Number 1.5 mL DNA-LoBind tubes Eppendorf 86–923 100 μm cell strainer Falcon 352–360 15 mL tubes VWR 525–1071 2X TD buffer Illumina 15027866 384-well PCR plate Applied biosystem 4483285 40 μm cell strainer Falcon 352–340 50 mL tubes VWR 525–1077 AMPure XP reagent (SPRI beads) Beckman Coulter A63881 Bioanalyzer High Sensitivity DNA kit Agilent Technologies 5067–4626 Clear adhesive film Applied biosystem 4306311 DNase/RNase-free distilled water Invitrogen 10977015 Dounce tissue grinder DWK Life Sciences 357542 DTT Sigma D9779 DynaMag-96 side skirted magnet Thermo Fishers 12027 FACS tubes Falcon 28719128 HEPES Boston BioProducts BBH-75 Hoechst 33342 Invitrogen 2134015 KCl (2 M) Boston BioProducts MT-252 Magnetic separation rack for PCR 8-tube strips EpiCypher 10–0008 MgCl 2 (1 M) Boston BioProducts MT-200 MinElute PCR purification kit Qiagen 28004 NEBNext High-Fidelity 2X PCR master mix BioLabs M0541S NP40 Thermo Fishers 28324 PCR 8-tube strip USA scientific 1402–4708 Protease inhibitor cocktail (100X) Thermo Fishers 78439 Qubit dsDNA HS assay kit Invitrogen Q32851 Sucrose Sigma S0389–1KG SYBR Green I (10,000X) Invitrogen S7563 TDE I enzyme Illumina 15027865 Instruments Name Company Catalog Number Flow cytometer BD Biosciences FACSAria TM Fusion Qubit fluorometer Invitrogen Q33226 Real-Time PCR system Thermo Fishers QuantStudio 5 Open in a separate window Table of Materials.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fishers , 12027.

    Article Title: Innate acting memory Th1 cells modulate heterologous diseases
    Article Snippet: Briefly, Agencourt RNAClean XP paramagnetic beads (Beckman Coulter) were used in combination with a DynaMag-96 side skirted magnet (Thermo Fisher). cDNA was generated with the SuperScript II Reverse Transcriptase Kit (Thermo Fisher) and amplified with HiFi HotStart PCR Mix (KAPA Biosystems).

    Article Title: Product and methods useful for modulating and evaluating immune responses
    Article Snippet: The lysate plate was transferred to a magnet (DynaMag-96 Side Skirted Magnet, Life Technologies), the supernatant was removed, and the beads were washed three times in 100 μL of 80% ethanol, with care being taken to avoid loss of beads during the washes.

    Article Title: Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fisher Scientific , 12027.

    Article Title: RNA methylation controls stress granule formation and function during erythropoiesis
    Article Snippet: End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25 0 C for 45 min. End-repaired DNA was purified using AMPure XP Beads (1.8X of the reaction volume) (Agencourt AMPure XP – PCR purification Beads, BeckmanCoulter, A63881) and separating beads using DynaMag-96 Side Skirted Magnet (Life Technologies, 12027).

    Diagnostic Assay:

    Article Title: Immune correlates of HIV-1 reservoir cell decline in early-treated infants
    Article Snippet: DynaMag-96 Side Skirted Magnet , ThermoFisher Scientific , 12027.

    Article Title: Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
    Article Snippet: Invitrogen DynaMag-96 side skirted magnet , Thermo Fisher Scientific , Cat# 12027.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: Animals Name Company Catalog Number Adiponectin-Cre mouse The Jackson Laboratory 28020 NuTRAP mouse The Jackson Laboratory 29899 Reagents & Materials Name Company Catalog Number 1.5 mL DNA-LoBind tubes Eppendorf 86–923 100 μm cell strainer Falcon 352–360 15 mL tubes VWR 525–1071 2X TD buffer Illumina 15027866 384-well PCR plate Applied biosystem 4483285 40 μm cell strainer Falcon 352–340 50 mL tubes VWR 525–1077 AMPure XP reagent (SPRI beads) Beckman Coulter A63881 Bioanalyzer High Sensitivity DNA kit Agilent Technologies 5067–4626 Clear adhesive film Applied biosystem 4306311 DNase/RNase-free distilled water Invitrogen 10977015 Dounce tissue grinder DWK Life Sciences 357542 DTT Sigma D9779 DynaMag-96 side skirted magnet Thermo Fishers 12027 FACS tubes Falcon 28719128 HEPES Boston BioProducts BBH-75 Hoechst 33342 Invitrogen 2134015 KCl (2 M) Boston BioProducts MT-252 Magnetic separation rack for PCR 8-tube strips EpiCypher 10–0008 MgCl 2 (1 M) Boston BioProducts MT-200 MinElute PCR purification kit Qiagen 28004 NEBNext High-Fidelity 2X PCR master mix BioLabs M0541S NP40 Thermo Fishers 28324 PCR 8-tube strip USA scientific 1402–4708 Protease inhibitor cocktail (100X) Thermo Fishers 78439 Qubit dsDNA HS assay kit Invitrogen Q32851 Sucrose Sigma S0389–1KG SYBR Green I (10,000X) Invitrogen S7563 TDE I enzyme Illumina 15027865 Instruments Name Company Catalog Number Flow cytometer BD Biosciences FACSAria TM Fusion Qubit fluorometer Invitrogen Q33226 Real-Time PCR system Thermo Fishers QuantStudio 5 Open in a separate window Table of Materials.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fishers , 12027.

    Article Title: Innate acting memory Th1 cells modulate heterologous diseases
    Article Snippet: Briefly, Agencourt RNAClean XP paramagnetic beads (Beckman Coulter) were used in combination with a DynaMag-96 side skirted magnet (Thermo Fisher). cDNA was generated with the SuperScript II Reverse Transcriptase Kit (Thermo Fisher) and amplified with HiFi HotStart PCR Mix (KAPA Biosystems).

    Article Title: Product and methods useful for modulating and evaluating immune responses
    Article Snippet: The lysate plate was transferred to a magnet (DynaMag-96 Side Skirted Magnet, Life Technologies), the supernatant was removed, and the beads were washed three times in 100 μL of 80% ethanol, with care being taken to avoid loss of beads during the washes.

    Article Title: Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fisher Scientific , 12027.

    Article Title: RNA methylation controls stress granule formation and function during erythropoiesis
    Article Snippet: End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25 0 C for 45 min. End-repaired DNA was purified using AMPure XP Beads (1.8X of the reaction volume) (Agencourt AMPure XP – PCR purification Beads, BeckmanCoulter, A63881) and separating beads using DynaMag-96 Side Skirted Magnet (Life Technologies, 12027).

    Digital PCR:

    Article Title: Immune correlates of HIV-1 reservoir cell decline in early-treated infants
    Article Snippet: DynaMag-96 Side Skirted Magnet , ThermoFisher Scientific , 12027.

    Article Title: Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
    Article Snippet: Invitrogen DynaMag-96 side skirted magnet , Thermo Fisher Scientific , Cat# 12027.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: Animals Name Company Catalog Number Adiponectin-Cre mouse The Jackson Laboratory 28020 NuTRAP mouse The Jackson Laboratory 29899 Reagents & Materials Name Company Catalog Number 1.5 mL DNA-LoBind tubes Eppendorf 86–923 100 μm cell strainer Falcon 352–360 15 mL tubes VWR 525–1071 2X TD buffer Illumina 15027866 384-well PCR plate Applied biosystem 4483285 40 μm cell strainer Falcon 352–340 50 mL tubes VWR 525–1077 AMPure XP reagent (SPRI beads) Beckman Coulter A63881 Bioanalyzer High Sensitivity DNA kit Agilent Technologies 5067–4626 Clear adhesive film Applied biosystem 4306311 DNase/RNase-free distilled water Invitrogen 10977015 Dounce tissue grinder DWK Life Sciences 357542 DTT Sigma D9779 DynaMag-96 side skirted magnet Thermo Fishers 12027 FACS tubes Falcon 28719128 HEPES Boston BioProducts BBH-75 Hoechst 33342 Invitrogen 2134015 KCl (2 M) Boston BioProducts MT-252 Magnetic separation rack for PCR 8-tube strips EpiCypher 10–0008 MgCl 2 (1 M) Boston BioProducts MT-200 MinElute PCR purification kit Qiagen 28004 NEBNext High-Fidelity 2X PCR master mix BioLabs M0541S NP40 Thermo Fishers 28324 PCR 8-tube strip USA scientific 1402–4708 Protease inhibitor cocktail (100X) Thermo Fishers 78439 Qubit dsDNA HS assay kit Invitrogen Q32851 Sucrose Sigma S0389–1KG SYBR Green I (10,000X) Invitrogen S7563 TDE I enzyme Illumina 15027865 Instruments Name Company Catalog Number Flow cytometer BD Biosciences FACSAria TM Fusion Qubit fluorometer Invitrogen Q33226 Real-Time PCR system Thermo Fishers QuantStudio 5 Open in a separate window Table of Materials.

    Article Title: Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fishers , 12027.

    Article Title: Innate acting memory Th1 cells modulate heterologous diseases
    Article Snippet: Briefly, Agencourt RNAClean XP paramagnetic beads (Beckman Coulter) were used in combination with a DynaMag-96 side skirted magnet (Thermo Fisher). cDNA was generated with the SuperScript II Reverse Transcriptase Kit (Thermo Fisher) and amplified with HiFi HotStart PCR Mix (KAPA Biosystems).

    Article Title: Product and methods useful for modulating and evaluating immune responses
    Article Snippet: The lysate plate was transferred to a magnet (DynaMag-96 Side Skirted Magnet, Life Technologies), the supernatant was removed, and the beads were washed three times in 100 μL of 80% ethanol, with care being taken to avoid loss of beads during the washes.

    Article Title: Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing
    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fisher Scientific , 12027.

    Article Title: RNA methylation controls stress granule formation and function during erythropoiesis
    Article Snippet: End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25 0 C for 45 min. End-repaired DNA was purified using AMPure XP Beads (1.8X of the reaction volume) (Agencourt AMPure XP – PCR purification Beads, BeckmanCoulter, A63881) and separating beads using DynaMag-96 Side Skirted Magnet (Life Technologies, 12027).



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    Image Search Results


    Journal: STAR Protocols

    Article Title: Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing

    doi: 10.1016/j.xpro.2024.103594

    Figure Lengend Snippet:

    Article Snippet: DynaMag-96 side skirted magnet , Thermo Fisher Scientific , 12027.

    Techniques: Recombinant, Plasmid Preparation, Reverse Transcription, DNA Ligation, Library Quantification, Sequencing, Software, Sterility, Adhesive